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KMID : 1145120210190010065
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Asian Journal of Beauty and Cosmetology
2021 Volume.19 No. 1 p.65 ~ p.76
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Suppression of Matrix Metallopeptidase-3 Expression in Human Dermal Fibroblasts by Decursin from Angelica gigas Nakai Root Extracts Fermented with Jeju Lava Seawater
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Kang Jung-Wook
Nam Ga-Ram
Yoon Yea-Hwa
Kim Gyeong-Mi
Bae So-Won
Lee Hyun-Jeong
Bae Seung-Hee
Cha Young-Kwon
Cho Hyun-Dae
Cho Hang-Eui
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Abstract
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Purpose : This study aims to understand the role Angelica gigas (A. gigas) Nakai root extract (AGNRE) fermented with Jeju lava seawater in collagenase suppression in human dermal fibroblasts, and identify the major active compound responsible for it suppressive effect.
Methods : AGNREs were prepared by fermentation with Jeju lava water at low temperature and analyzed for identifying the major active compound in these fermented root extracts using high-performance liquid chromatography (HPLC). Water-soluble tetrazolium salt (WST-1) assay and real-time polymerase chain reaction (qRT-PCR) were performed to investigate the cell viability and expression level of collagenase gene. Comparative experiments were performed using AGNREs and decursin to confirm the downregulation of collagenase expression and analyze the correlation between them.
Results : HPLC analysis revealed decursin to be the major active compound in AGNREs. Analysis of data obtained by WST-1 assays at concentration of 200 μg/mL for AGNREs and <20 μM for decursin did not show any cytotoxicity in human dermal fibroblasts. qRT-PCR analysis revealed that AGNREs as well as decursin downregulated the expression of matrix metalloproteinases-3 (MMP3) gene in human dermal fibroblasts.
Conclusion : These analyses suggest that AGNREs fermented with Jeju lava water as well as decursin isolated from these extracts hold a great potential to be applied as functional anti-wrinkle agent in cosmetic.
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KEYWORD
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Angelica gigas Nakai, Decursin, Collagenase, Jeju lava seawater, Dermal fibroblasts
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